Duolink Image Tools 1.0

Duolink Image Tools is a specialized image analysis solution designed for researchers working with Duolink proximity ligation assay (PLA) microscopy data. It streamlines the detection and quantification of punctate PLA signals, helping you turn fluorescence images into reproducible, per-cell measurements with minimal manual effort.

Key features

• PLA spot detection: Identify and count fluorescent puncta with adjustable thresholds and size filters.
• Per-cell quantification: Segment nuclei/cells (e.g., via DAPI) to report spots per cell, density, and intensity statistics.
• Batch processing: Apply consistent analysis settings across large image sets for higher throughput.
• Visual overlays: Preview detections and segmentation as overlays for quick quality control.
• Flexible inputs/outputs: Works with common microscopy image formats and exports results, summaries, and logs to CSV/Excel-friendly files.
• Reproducibility: Save and reuse analysis templates to standardize studies across experiments and users.

Ideal for

• Cell and molecular biologists analyzing protein–protein interactions or post-translational modifications via PLA.
• Core facilities and labs needing consistent, scalable quantification across imaging campaigns.
• Projects that require transparent, auditable pipelines for publication or validation.

Typical workflow

1. Import fluorescence micrographs.
2. Define channels and adjust detection parameters.
3. Segment nuclei/cells and preview overlays.
4. Run analysis (single images or batches).
5. Export per-cell and aggregate metrics for downstream statistics or visualization.

Note: Specific capabilities may vary by version; consult the tool’s documentation for detailed requirements and supported formats.

Duolink Image Tools is developed by Olink. The most popular version of this product among our users is 1.0. The name of the program executable file is ImageTool.exe.